home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9409d.zip
/
M9490556.TXT
< prev
next >
Wrap
Text File
|
1994-09-24
|
2KB
|
35 lines
Document 0556
DOCN M9490556
TI [Contribution of gene amplification in the biological diagnosis of
toxoplasmosis]
DT 9411
AU Cazenave J; Broussin B; Verin P; Tirard V; Cheyrou A; Begueret J;
Laboratoire de Biologie moleculaire, LABM, Bordeaux.
SO Presse Med. 1994 Mar 26;23(12):573-5. Unique Identifier : AIDSLINE
MED/94344928
AB Biological diagnosis of toxoplasmosis is generally based on indirect
arguments (serology). In cases of immaturity or of immune depression,
however, evidence of the parasite has to be obtained. This involves
time-consuming or relatively insensitive culture techniques. Molecular
biology, and more particularly the polymerase chain reaction gene
amplification technique, makes it possible to identify an extremely
small quantity of parasites in a complex biological fluid in a few
hours. We summarized our experience with an original technique using
toxoplasma ribosomal DNA as the target. Its use in the amniotic fluid
provides a distinct improvement in antenatal diagnosis and is fast
becoming the technique of reference. Difficult cases have been solved by
its application in ophthalmology. Finally, in immunodepressed patients,
especially in cases of acquired immunodeficiency syndrome, assessment is
currently ongoing. The results obtained so far, especially in the
analysis of the cerebral spinal fluid, are encouraging.
DE Adult English Abstract Human Immune Tolerance Infant, Newborn
Polymerase Chain Reaction/*METHODS Prenatal Diagnosis
Toxoplasmosis/*DIAGNOSIS/GENETICS Toxoplasmosis,
Cerebral/*DIAGNOSIS/GENETICS Toxoplasmosis,
Congenital/*DIAGNOSIS/GENETICS Toxoplasmosis,
Ocular/*DIAGNOSIS/GENETICS JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).